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浙江大学学报（理学版）
浙江大学学报（理学版）  2016, Vol. 43 Issue (2): 220-225    DOI: 10.3785/j.issn.1008-9497.2016.02.017
化学     
柱切换在线前处理法鉴别地沟油的离子色谱研究
赵训燕1,2, 倪承珠1,2, 朱彬和1,2, 张培敏1, 张嘉捷1, 陈素清3, 朱岩1,2
1. 浙江大学化学系, 浙江杭州 310028;
2. 浙江大学浙江省微量有毒化学物健康风险评估技术研究重点实验室, 浙江杭州 310028;
3. 台州学院制药与化学工程学院, 浙江台州 317000
Distinguishing illegal cooking oils by ion chromatography with column-switching on-line sample pretreatment
ZHAO Xunyan1,2, NI Chengzhu1,2, ZHU Binhe1,2, ZHANG Peimin1, ZHANG Jiajie1, CHEN Suqing3, ZHU Yan1,2
1. Department of Chemistry, Zhejiang University, Hangzhou 310028, China;
2. Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Zhejiang University, Hangzhou 310028, China;
3. School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 317000, Zhejiang Province, China
 全文: PDF(989 KB)  
摘要： 建立了同时检测食用油及地沟油中的无机阴离子(F-、Cl-、SO42-)和有机酸(CH3CO2-、CHO2-)的离子色谱柱切换在线前处理方法.采用一根NG1反相色谱柱作为前处理柱在线去除样品中的水溶性有机基质,通过柱切换技术,由淋洗液将柱内富集的离子带至阴离子分析柱中分离,从而达到在线前处理的目的.在优化的色谱条件下,被分析离子浓度在0.05~10 mg·L-1时,线性相关系数均大于0.9990;进样量25 μL,方法的检出限为4~41 μg·L-1(信噪比S/N=3);加标回收率在75.6%~87.7%;相对标准偏差在0.85%~2.34%(n=6);实验结果表明,地沟油中甲酸和乙酸的含量明显高于食用油.本方法对食用油和地沟油的鉴别具有较好的实用价值,适用于食用油及地沟油中常规阴离子和有机酸的同步检测,具有快速、灵敏、选择性好等优点.
关键词： 离子色谱;  柱切换;  常规阴离子;  有机酸    
Abstract: The ion chromatography with on-line sample pretreatment using column-switching technique is applied to the simultaneous analysis of the conventional inorganic anions and organic acids in edible oils and illegal cooking oils. In this work, a polymer-based reversed-phase column was used as the pretreatment column. Via column-switching technique, sample solution with target ions were eluted from a collection loop to the analytical columns with matrix eliminated on-line. A series of standard solutions consisting of target ions of various concentrations from 0.05 to 10 mg·L-1 are analyzed, with a correlation coefficient r≥0.9990 and a satisfactory repeatability (RSD≤2.34%, n=6). The limits of detection are in the range from 4 to 41 μg·L-1 based on S/N of 3 and the 25 μL injection volume. The average spiked recovery rates are between 75.6% and 87.7%. The result shows that the concentration of acetate and formate are significantly higher in the illegal cooking oils than edible oils. The on-line sample pretreatment IC system is convenient and practical for the determination of conventional inorganic anions and organic acids in edible and illegal cooking oil samples.
Key words: ion chromatography    column-switching    conventional inorganic anions    organic acids
收稿日期: 2015-06-23 出版日期: 2016-03-12
CLC:  O657.7  
基金资助: 国家重大科学仪器设备开发专项(2012YQ09022903);浙江省自然科学基金资助项目(Y12B050014,LQ13B050001,LY12B05003).
通讯作者: 朱岩,E-mail:zhuyan@zju.edu.cn.     E-mail: zhuyan@zju.edu.cn
作者简介: 赵训燕(1991-),ORCID:http://orcid.org/0000-0002-9731-991X,女,硕士研究生,主要从事离子色谱研究.
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引用本文:

赵训燕, 倪承珠, 朱彬和, 张培敏, 张嘉捷, 陈素清, 朱岩. 柱切换在线前处理法鉴别地沟油的离子色谱研究[J]. 浙江大学学报（理学版）, 2016, 43(2): 220-225.

ZHAO Xunyan, NI Chengzhu, ZHU Binhe, ZHANG Peimin, ZHANG Jiajie, CHEN Suqing, ZHU Yan. Distinguishing illegal cooking oils by ion chromatography with column-switching on-line sample pretreatment. Journal of ZheJIang University(Science Edition), 2016, 43(2): 220-225.

链接本文:

https://www.zjujournals.com/sci/CN/10.3785/j.issn.1008-9497.2016.02.017    或    https://www.zjujournals.com/sci/CN/Y2016/V43/I2/220

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