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浙江大学学报（理学版）
浙江大学学报（理学版）  2016, Vol. 43 Issue (2): 195-199    DOI: 10.3785/j.issn.1008-9497.2016.02.013
生命科学     
纳米银对荧光假单胞菌生物膜形成的影响
黄迅辰1,2, 杨维1,2, 戴贤君1,2
1. 中国计量学院生命科学学院, 浙江杭州 310018;
2. 浙江省海洋食品品质及危害物控制技术重点实验室, 浙江杭州 310018
Effect of silver nanoparticles on biofilm formation of Pseudomonas fluourcens
HUANG Xunchen1,2, YANG Wei1,2, DAI Xianjun1,2
1. College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
2. Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, Hangzhou 310018, China
 全文: PDF(1122 KB)  
摘要： 分别以空白和0.4%纳米银乙烯醋酸乙烯酯(EVA)塑料片作为载体培养荧光假单胞菌生物膜,通过菌落计数法测定生物膜的变化曲线,分别采用普通显微镜观察生物膜形成并计算覆盖率(BCR),扫描电镜观察生物膜的微观结构,激光共聚焦显微镜观察生物膜中的细菌状态并计算死亡率.结果表明,空白、纳米银EVA表面生物膜分别在12和24 h达到稳定状态,各时间点的纳米银EVA表面菌数及BCR均显著低于空白EVA(p<0.05);在10000倍的视野下,空白EVA表面的荧光假单胞菌通过胞外产物和菌体形成致密生物膜,纳米银EVA表面多为分散菌体;在24,48 h时,纳米银EVA表面的死亡菌体数显著高于空白EVA(p<0.05);纳米银通过抑制荧光假单胞菌生长和减少胞外产物的分泌而影响生物膜的形成.
关键词： 纳米银;  荧光假单胞菌;  生物膜    
Abstract: To explore the effect of silver nanoparticles on the formation of Pseudomonas fluourcens biofilms on ethylene-vinyl acetate(EVA) pieces, the curve of bacterial biofilm is determined by colony counts, and the morphology, microstructure and bacterial status of the biofilm are respectively observed through crystal voilet staining, scanning electron microscopy(SEM) and confocal laser scanning microscope(CLSM).The biofilm stained by crystal violet is examined with a digital microscope to calculate the biofilm coverage rate (BCR) by NIS-Element BR soft ware. Then the mortality of bacterial is calculated by image structure analyzer (ISA) software.Results show that the bacteria can aggregate on the control EVA and EVA contained 0.4% silver nanoparticles, and forms the grown biofilm after 12 and 48 h culture, respectively. But the biofilm on the control EVA has more bacterial count and BCR than that of silver nanoparticles(p<0.05). At 24 h, the bacteria interacting with its extracellular products can form the dense film in the microscope vision of 10000 times on the control EVA, while there are only dispersive bacteria on silver nanoparticle EVA. The bacterial mortality in the film on silver nanoparticle EVA is significantly higher than that of control group at 24, 48 h (p<0.05). Silver nanoparticles have great inhibiting effect on biofilm formation of Pseudomonas fluourcens through inhibiting the growth of the bacterial and its extracellucar products.
Key words: silver nanoparticles    Pseudomonas fluourcens    biofilms
收稿日期: 2015-03-31 出版日期: 2016-03-12
CLC:  R378.11  
基金资助: 浙江省重大科技专项资助项目(2012C03009-2).
通讯作者: 戴贤君,ORCID:http://orcid.org/0000-0002-8361-5187,E-mail:xjdai@cjlu.edu.cn.     E-mail: xjdai@cjlu.edu.cn
作者简介: 黄迅辰(1990-),男,硕士研究生,主要从事食品微生物研究.
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引用本文:

黄迅辰, 杨维, 戴贤君. 纳米银对荧光假单胞菌生物膜形成的影响[J]. 浙江大学学报（理学版）, 2016, 43(2): 195-199.

HUANG Xunchen, YANG Wei, DAI Xianjun. Effect of silver nanoparticles on biofilm formation of Pseudomonas fluourcens. Journal of ZheJIang University(Science Edition), 2016, 43(2): 195-199.

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https://www.zjujournals.com/sci/CN/10.3785/j.issn.1008-9497.2016.02.013    或    https://www.zjujournals.com/sci/CN/Y2016/V43/I2/195

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